Transgenic Mouse User Fees and Policies

The following policies are in place for the Transgenic Mouse shared resource relating to scheduling services and charges for use of the facility.

Service Request
Investigators who would like to request services from the facility should log in to iLab at https://cumc.corefacilities.org with their UNI and password, select Transgenic Mouse fromthe list of cores, and open the Request Services tab. Follow the instructions to initiate a request and fill int he appropriate forms. Contact the TNSR at cls5@cumc.columbia.edu if additional help is needed inmaking a request. Our services are provided based on the order in which the request is received.

User Fees

Pronuclear Injection
Cancer Center Member Pricing (for each DNA construct or clone) $2,156.00
Non-Cancer Center Member Pricing (for each DNA construct or clone) $2,800.00
External Pricing (for each DNA construct or clone) $ 4,480.00

Pronuclear Injection (C57BL/6J)
Cancer Center Member Pricing (for each DNA construct or clone) $5,818.89
Non-Cancer Center Member Pricing (for each DNA construct or clone) $7,557.00
External Pricing (for each DNA construct or clone) $12,091.20

Blastocyst Injection
Cancer Center Member Pricing (for each DNA construct or clone) $1,155.00
Non-Cancer Center Member Pricing (for each DNA construct or clone)$1,500.00
External Pricing (for each DNA construct or clone) $2,400.00

Thawing of Clones(1)
Cancer Center Member Pricing (for each DNA construct or clone) $102.41
Non-Cancer Center Member Pricing (for each DNA construct or clone)$133
External Pricing (for each DNA construct or clone) $212.80

CRISPR Service
Cancer Center Member Pricing (for each DNA construct or clone) $2,156.00
Non-Cancer Center Member Pricing (for each DNA construct or clone)$2800.00
External Pricing (for each DNA construct or clone) $4,480.00

CRISPR (C57BL/6J) Service
Cancer Center Member Pricing (for each DNA construct or clone) $7557.00
Non-Cancer Center Member Pricing (for each DNA construct or clone)$7557.00
External Pricing (for each DNA construct or clone) $12,091.20

CRISPR Guide Service
Cancer Center Member Pricing $624.00
Non-Cancer Center Member Pricing $750.00
External Pricing $1200.00

BAC Purification
Cancer Center Member Pricing (for each DNA construct or clone) $1,000.00
Non-Cancer Center Member Pricing (for each DNA construct or clone)$1,250.00
External Pricing (for each DNA construct or clone) $2,000.00

ES Cell Electroporation
Cancer Center Member Pricing (for each DNA construct or clone) $4,004.00
Non-Cancer Center Member Pricing (for each DNA construct or clone)$5,200.00
External Pricing (for each DNA construct or clone) $8,320.00

Expansion of ES Cells for First Clone
Cancer Center Member Pricing (for each DNA construct or clone) $1,058.75
Non-Cancer Center Member Pricing (for each DNA construct or clone)$1,375.00
External Pricing (for each DNA construct or clone) $2,200.00

Additional Clones for the Same Experiment(2)
Cancer Center Member Pricing (for each DNA construct or clone) $96.25
Non-Cancer Center Member Pricing (for each DNA construct or clone) $125
External Pricing (for each DNA construct or clone) $200.00

ES Cell Derivation (setup charge)
Cancer Center Member Pricing (for each DNA construct or clone) $1,727.11
Non-Cancer Center Member Pricing (for each DNA construct or clone)$2,243.00
External Pricing (for each DNA construct or clone) $3,588.80

ES Cell Derivation (per ES cell line)
Cancer Center Member Pricing (for each DNA construct or clone) $146.30
Non-Cancer Center Member Pricing (for each DNA construct or clone)$190.00
External Pricing (for each DNA construct or clone) $304.00

Full Gene Targeting
Cancer Center Member Pricing $25,000.00
Non-Cancer Center Member Pricing $27,000.00
External Pricing (for each DNA construct or clone) $43,200.00

Notes On Services

  1. Thawing of frozen ES clones for microinjection will incur a charge for each clone thawed at a rate of $100 (CC Members) or $125 per clone (Non-Members).
  2. After the first clone of ES Cells, each additional clone for the same experiment will be $100 (CC Members) or $125 (Non-Members).
  3. TMSR will expand each ES line to the well of 24-well plate and freeze two vials for each ES line. 50ul ES lysate will be provided for genotyping purpose. Setup charge will be waived if no ES line is obtained.

Other Policies

  • DNA samples and ES cell lines are injected in the order in which the signed service request forms are received. Currently, the average time from receipt of the service request to injection is approximately 2 weeks.
  • For ES Cell electroporation and expansion, success in gene targeting cannot be guaranteed due to inherent variability in the frequency of targeting different loci, and the possibility of mistakes or mutations in the targeting vector.