Flow Cytometry Instrumentation

Analyzers

Instrument Features Location
BD Fortessa 405, 488, and 633nm lasers, 14 colors
Configuration Chart
BB1710
BD Fortessa 405, 488, 561, and 633nm, 16 colors
Configuration Chart
ICRC 333
BD LSRII UV, 405, 488, 532, 594, and 633nm lasers, 18 colors
Configuration Chart
Non-default dye filter change suggestion
BB1710
BD LSRII UV, 405, 488, and 633nm, 18 colors
Configuration Chart
ICRC 333
BD FACSCanto II 405, 488, and 633nm lasers, 8 colors
Configuration Chart
P&S17-503

Cell Sorters

Instrument Features Location
BD Influx 405, 488, 561, and 633 lasers, 15 colors
Configuration Chart
Self Operation Policy
BB1710
BD Influx 405, 488, 561, and 633 lasers, 15 colors
Configuration Chart
Self Operation Policy
P&S 17-503
Aria II 405, 488, 561, and 633 nm, 14 colors
Configuration Chart
ICRC 312

Sample Preparation Guidelines for analyzers and sorters.

Leica DMI 6000BB: Configuration chart

Analysis Workstations:

Workstations in PS17-503 and ICRC 333 with FCS Express, Flowjo and Microsoft Office installed are available to all the core users for free. Please sign up through the ilab.

Flow Analysis Data Packages

  1. FlowJo: Please contact Siu-Hong Ho (sh3056@columbia.edu) to set up a site license. Be advised: FlowJo site licensing will be terminated on 2/28/2018.
  2. FCS Express: Please contact Siu-Hong Ho (sh3056@columbia.edu) to set up a free trial, which will last until 3/31/18. Users can then purchase a license for FCS Express after 3/31/2018.