Researcher’s Profile

Jean Gautier, PhD

Leader, Cancer Genetics and Epigenetics Program, Herbert Irving Comprehensive Cancer Center
Associate Director of Research, Herbert Irving Comprehensive Cancer Center
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The laboratory employs diverse experimental approaches to elucidate the role of genome instability in cancer. Cell-free extracts derived from the egg of the frog Xenopus laevis are used as a simple model system to study processes that govern genome stability, including DNA replication control, DNA repair, and the cellular response to DNA damage. In addition, cultured mammalian cells and mouse models are exploited to analyze biological responses to DNA damage. Several specific questions are currently being addressed in his lab. First, what repair pathways are involved in processing DNA lesions induced by cancer chemotherapeutic drugs? Specifically, Dr. Gautier’s lab examines double-strand break repair and DNA inter-strand crosslink repair. The second is, what conditions favor mis-repair of DNA and lead to chromosome rearrangements? The third is, what are the molecular origins of oncogene-induced genomic stress? 

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The main objective of our research is to better understand the molecular mechanisms responsible for the maintenance of genome stability. These controls are lost in cancer, which is characterized by genomic instability.

Research Statement: 

The laboratory employs diverse experimental approaches to elucidate the role of genome instability in cancer. Cell-free extracts derived from the egg of the frog Xenopus laevis are used as a simple model system to study processes that govern genome stability, including DNA replication control, DNA repair, and the cellular response to DNA damage. In addition, cultured mammalian cells and mouse models are exploited to analyze biological responses to DNA damage. Several specific questions are currently being addressed in his lab. First, what repair pathways are involved in processing DNA lesions induced by cancer chemotherapeutic drugs? Specifically, Dr. Gautier’s lab examines double-strand break repair and DNA inter-strand crosslink repair. The second is, what conditions favor mis-repair of DNA and lead to chromosome rearrangements? The third is, what are the molecular origins of oncogene-induced genomic stress? 

Lab Members: 
Tomas Aparicio, Research Associate
Niyo Kato, Graduate Student
Andrew Liebau, Graduate Student
Mai Sato, Post-doctoral Fellow
Benjamin Schrank, MD/PhD Student
Gaganpreet Sidhu, Post-doctoral Fellow
Tao Yu, Post-doctoral Fellow
Soumini Vasan, Laboratory Manager
Crystal Waters, Post-doctoral Fellow
Publications: 

Williams H., Gottesman M. and Gautier J. (2012). Replication-independent repair of interstrand crosslinks. Molecular Cell, 47: 140-7.

Peterson, S., Li, Y., Wu-Baer, F., Chait, B., Baer, R., Yan, H., Gottesman, M. and Gautier J. (2013). Activation of DSB processing requires phosphorylation of CtIP by ATR. Molecular Cell, 49: 657-67.

Srinivasan, S., Dominguez-Sola, D., Wang, L.C., Hyrien, O. and Gautier, J. (2013) “Cdc45 is a critical effector of Myc-dependent DNA replication stress”. Cell Reports, 3:1629-39.

Sato, M., Rodriguez-Barrueco R., Yu J., Do C., Silva JM and Gautier J. (2015) “MYC is a critical target of FBXW7. Oncotarget 6: 3292-305.

Aparicio, T., Baer, R., Gottesman, M. and Gautier, J. (2016) “MRN, CtIP, and BRCA1 mediate repair of topoisomerase II–DNA adducts”. The Journal of Cell Biology. 212: 399-408.

Kato N., Kawasoe Y., Williams H., Coates E., Roy U., Shi Y., Beese L., Scharer O., Yan H., Gottesman M., Takahashi T. and Gautier J. (2017) Sensing and processing of DNA interstrand crosslinks by the mismatch repair pathway. Cell Reports. 21: 1375-1385.
Link to complete list of publications:: https://scholar.google.com/citations?user=OGR--C0AAAAJ&hl=en&oi=ao