Donna L. Farber, PhD
Immune memory and protective immunity Influenza immune responses Transplantation immunology Autoimmunity
Research in our laboratory is focused on immunological memory and specifically on memory T cells as essential mediators of protective immunity. While it was previously thought that memory T cells mediate their protective responses through rapid migration and surveillance through tissues, it is now become clear that localization and establishment of non-circulating memory T cells resident in tissue sites is integral to immune protection. We are incorporating fundamental studies on mouse models with novel translational approaches on human samples to investigate tissue immune responses. We have identified a new subset of non-circulating tissue-resident memory T cells (TRM) in the lung that mediate optimal protective immunity in a mouse model of influenza infection. Current studies into mechanisms for how memory T cells become targeted to and maintained in the lung use total transcriptome profiling and bioinformatics approaches. We have identified novel roles for specific integrins and inflammatory mediators in this process, and are studying the signaling pathways involved in resident memory T cell generation and functional recall. For our translational studies, we have established a unique collaboration and research protocol with the organ procurement organization for the New York Metropolitan area (LiveOnNY ; http://www.donatelifeny.org/) and transplant surgeons at New York Presbyterian (NYP) where we obtain multiple lymphoid and mucosal tissues from research-consented human organ donors. These studies are part of an NIH-funded Program on “Tissue compartmentalization of human lymphocytes”, involving immunologists, molecular biologists and computational biologists in five institutions to study human adaptive and innate lymphocyte compartmentalization and maintenance in human tissues throughout the human lifespan. Additionally, because memory T cells are critically important to generate in vaccines, we have ongoing studies on infant immunity, to investigate how protective responses can be established in babies who are most susceptible to infection and immune pathologies. These infant studies involve both mouse models and also sampling of site-specific responses in human infants intubated due to virus infection in collaboration with clinicians in the Pediatric Critical care Division at Morgan Stanley/NYP.
Teijaro, J.R., Turner, D., Nam, Q., Wherry, E.J., LeFrançois, L. and Farber, D.L. (2011) Cutting Edge: Tissue-retentive lung memory CD4 T cells mediate optimal protective responses to influenza. J. Immunol. 187:5510-14.
Sathaliyawala, T., Kubota, M., Yudanin, N., Turner, D., Camp, P., Thome, J.C., Bickham, K.L., Lerner, H., Goldstein, M., Sykes, M., Kato, T and Farber, D.L. (2013) Distribution and compartmentalization of human circulating and tissue-resident memory T cells. Immunity. 38: 187-197. (Also see preview article in same issue by Neuenhahn and Busch, D.H., “Whole body anatomy of human T cells”. Immunity. 38:10-12.)
Turner, D.L., Bickham, K.L., Farber, D.L. and Lefrancois, L. (2013) Splenic priming of virus-specific CD8 T cells following influenza infection. J. Virol. 87:4496-506. Turner, D.L., Bickham, K.L., Thome, J.J.T., Kim, C., D’Ovidio, F., Wherry, E.J. and Farber, D.L. (2014) Lung resident niches for the generation and maintenance of influenza-specific memory T cells. Mucosal Immunology. 7:501-10.
Farber, D.L. , Yudanin, N.A. and Restifio, N.P. (2014) Human Memory T cells: generation, compartmentalization and homeostasis. Nature Reviews Immunology.14:24-35. (featured on cover) PMCID:PMC4032067
Turner, D.L., Gordon, C.L. and Farber, D.L. (2014) Tissue resident T cells, in situ immunity and transplantation. Immunol. Reviews. 258:150-166. Zens, K.D. and Farber, D.L. (2015) Memory CD4 T cell responses to influenza virus. Current Topics in Microbiology and Immunology. 386:399-421.
Turner, D.L. and Farber, D.L. (2014) Mucosal resident memory CD4 T cells in protection and immunopathology. Front. Immunol. 5:331 Free article
Thome, J.J.C., Yudanin, N.A., Ohmura, Y., Kubota, M., Grinshpun, B., Sathaliyawala, T., Kato, T., Lerner, H., Shen, Y. and Farber, D.L. (2014) Spatial map of human T cell compartmentalization and maintenance over decades of life. Cell. 159:814-28. PMCID:PMC4243051
Brestoff,J.R., Kim,B.S., Saenz,S.A., Stine,R.R., Monticelli,L.A., Sonnenberg,G.A., Thome,J.J.T., Farber,D.L., Lutfy,K., Seale, P., and Artis, D. (2015) Group 2 innate lymphoid cells promote beiging of white adipose tissue and limit diet-induced obesity. Nature, 519:242-6. PMCID:PMC4447235
Thome, J.J.C. and Farber, D.L. (2015) Emerging concepts in tissue-resident T cells: lessons from humans. Trends. Immunol. 36:428-35. PMCID:PMC4491028
Yu, M., Owens, D.M., Ghosh, S. and Farber, D.L. (2015) Conditional PDK1 ablation promotes epidermal and T cell-mediated dysfunctions leading to inflammatory skin disease. J. Invest. Derm. 135: 2688-96. PMCID:PMC4640961
Thome, J.J.C., Bickham, K.L., Kubota, M. Ohmura, Y., Matsuoka, N., Gordon, C., Granot, T., Griesemer, A., Lerner, H., Kato, T., and Farber, D.L. (2016) Early-life compartmentalization of T-cell differentiation and regulatory function in mucosal and lymphoid tissues. Nature Medicine. 22:72-77. PMCID:PMC4703455
Trischler, J., Shiomi, T., Turner, D.L., Sklepkiewicz, P.L., Goldklang, M.P., Farber, D.L. and D’Armiento, J.M. (2016) Immune Modulation of the T cell Response in Asthma through Wnt10b. Amer. J. Resp. Cell and Mol. Biol. 54:584-93.
Zuber, J., Rosen, S., Shonts, B., Sprangers, B., Savage, T., Richman, S., Yang, S., Lau, S.P., DeWolf, S., Farber, D.L., Vlad, G., Zorn, E., Wong, W., Emond, J., Levin, B., Martinez, M., Kato, T., Sykes, M. (2015) Macrochimerism in intestinal transplantation: association with lower rejection rates and multivisceral transplants, without GVHD. Am. J. Transplant. 15:2691-703.
Connors, T.J., Ravindranath,T.M., Bickham,K.L., Gordon,C.L., Zhang, F., Levin,B., Baird, J.S. and Farber, D.L. (2016) Airway CD8 T-cells are associated with lung injury during infant viral respiratory tract infection. Amer. J. Resp. Cell and Mol. Biol. 54:822-830.
Farber,D.L., Netea,M., Radbruch,A., Rajewsky,K. and Zinkernagel, R. (2016) Immunological memory: lessons from the past and a look to the future. Nat. Rev. Immunol. 16: 124-8.
Connors, T.J., Baird, J. and Farber, D.L. (2016) Viral Bronchiolitis in children (correspondance) N. Engl. J. Med. 374:1791-2.
Zens, K.D., Chen, J.-K. and Farber, D.L. (2016) Vaccine-generated lung tissue-resident memory T cells provide heterosubtypic protection to influenza virus infection. J. Clin, Invest. Insight, 1(10):e85832. doi:10.1172/jci.insight.85832. PMCID: PMC4959801
Murray, A.J., Kwon, K.J., Farber, D.L. and Siliciano, R.F. (2016) The latent reservoir for HIV-1: how immunologic memory and clonal expansion contribute to HIV persistence. J. Immunol. 197:407-417. PMCId:PMC4936486
Zuber J., Shonts, B., Lau, S.-P., Obradovic, A., Fu, J., Yang, S., Lambert, M., Coley, S., Weiner, J., Thome, J.J.C., DeWolf, S., Farber, D.L., Shen, Y., Caillat-Zucman, S., Bhagat, G., Griesemer,A., Martinez,M., Kato, T. and Sykes,M. (2016) Bidirectional intragraft alloreactivity drives the repopulation of human intestinal allografts and correlates with clinical outcome. Sci. Immunol. 1:eaah3732. PMCID:PMC5323244
Thome, J.J.C., Grinshpun, B., Kumar, B.V., Kubota, M., Ohmura, Y., Lerner, H., Sempowski, G.D., Shen, Y. and Farber, D.L. (2016) Longterm maintenance of human naive T cells through in situ homeostasis in lymphoid tissue sites. Sci. Immunol.1: eaah6506. PMCID: PMC5367636
Nish, S, Zens, K. D. Kratchmarov, R., Lin,W.-H., Adams,W., Chen,Y.-H., Yen,B., Rothman, N., Bhandoola,A., Xue,H.-H., Farber, D.L. and Reiner, S. (2017) CD4+ T Cell Effector Commitment Coupled to Self-renewal by Asymmetric Cell Divisions. J. Exp. Med. 214:39-47. PMCID:PMC5206501
Gordon, C.L., Miron, M., Thome, J.J.C., Matsuoka, N., Weiner, J., Rak, M., Igarashi, S., Granot, T., Lerner, H., Goodrum, F. and Farber, D.L. (2017) Tissue-reservoirs of T cell immunity in persistent human CMV infection. J. Exp. Med. 214:651-667. PMCID: PMC5339671
Weiner, J., Farber, D.L., Kato, T. and Sykes, M. (2017) Long-term persistence of innate lymphoid cells in the gut after intestinal transplantation. Transplantation, [Epub ahead of print] PMID:27941430
Granot, T., Senda, T., Carpenter, D., Matsuoka, N., Weiner, J., Gordon, C.L., Miron, M., Kumar, B.V., Griesemer, A., Ho, S.-H., Lerner, H., Thome, J.J.C., Connors, T., Reizis, B., and Farber, D.L. (2017) Dendritic cells display subset and tissue-specific maturation dynamics over human life. Immunity, 46:504-515. PMCID:PMC5415308
Meng, W., Zhang, B., Schwartz, G.W., Rosenfeld, A.M., Ren, D., Thome, J.J.C., Carpenter, D., Matsuoka, N., Granot, T., Farber, D.L., Shlomchik, M.J., Hershberg, U. and Luning Prak, N.T. (2017) An atlas of B cell clonal distribution in the human body. Nat. Biotech. In Press.
Yocum, G., Turner, D.L., Danielsson, J. Barajas, M., Zhang, Y., Xu, D. Harrison, N., Homanics, G., Farber, D.L., and Emala, C. (2017) GABAA Receptor α4 Subunit Knockout Enhances Lung Inflammation and Airway Reactivity in a Murine Asthma Model. Am. J. Physiol.-Lung Cell. Mol. Physiol.doi: 10.1152/ajplung.00107.2017 (epub ahead of print).
Snyder, M.E. and Farber, D.L. (2017) Thymic-peripheral crosstalk in lymphoodepletion therapy. Am. J. Trans. 17:1970-1971.
Klose, C.S.N., Mahlakõiv, T., Moeller, J. B., Rankin, L.C., Flamar, A.-L., Kabata, H., Monticelli, L.A., Moriyama, S., Garbès Putzel, G., Rakhilin, N., Shen, X., Kostenis, E., König, G.M., Senda, T., Carpenter, D., Farber, D.L. and Artis, D. (2017) The neuropeptide neuromedin 2 stimulates innate lymphoid cells and promotes type 2 inflammation. Nature: In Press.
Turner, D.L., Goldklang, M., Trischler, J., Cvetkovski, F., D’Armiento, J.M., and Farber, D.L. (submitted) Biased Generation and in situ activation of lung tissue-resident memory CD4 T cells in the pathogenesis of allergic asthma. J. Immunol. In revision.
Kumar, B.V., Ma, W. Miron, M., Granot, T., Guyer, R.S., Carpenter, D.J., Senda, T., Ho, S.-H., Lerner, H., Friedman, A.L., Shen, Y., Farber, D.L. (2017) Human tissue-resident memory T cells are defined by core transcriptional and functional signatures in lymphoid and mucosal sites. Cell Reports. In Press.
Zens, K.D., Chen, J.-K., Guyer, R.S., Wu, F.L., Cvetkovski, F., Miron, M. and Farber, D.L. (2017) Reduced generation of lung-tissue resident memory T cells during infancy. J. Exp. Med. In Press.
Carpenter, D.J., Matsuoka, N., Granot, T., Kumar, B.V., Senda, T., Thome, J.J.C., Gordon, C.L., Miron, M., Weiner, J., Lerner, H., Friedman, A., Griesemer, A.D., Farber, D.L. (2017) Human immunology studies using organ donors: impact of clinical variations on immune parameters in tissues and circulation. Am. J. Trans. doi: 10.1111/ajt.14434. [Epub ahead of print].
Zens, K.D., Connors, T., and Farber, D.L. (2017) Tissue compartmentalization of T cell responses during early life. Semin. Immunopath. In Press.
Kumar, B.V., Connors, T. and Farber, D.L. (submitted) Human T cells in health and homeostasis. Immunity.